Stabilized reverse transcriptase fusion proteins

Details Stabilized reverse transcriptase fusion proteins Stabilized reverse transcriptase fusion proteins

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) C12N 9/12 (2006.01) C12N 9/96 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2754476

Stabilized reverse transcriptase fusion proteins including a thermostable reverse transcriptase connected to a stabi-lizer protein are described. Attaching the stabilizer protein to the thermostable reverse transcriptase stabilizes the fusion protein and can aid in its purification, provide increased solubility, allow for longer storage, or allow the fusion protein to be used under more rigorous conditions such as higher temperature. The stabilized reverse transcriptase fusion protein can also include a linker between the stabilizer protein and the thermostable reverse transcriptase. The stabilized reverse transcriptase fusion proteins are suitable for use in nucleic acid amplification methods such as the reverse transcription polymerase chain reaction and other appli-cations involving cDNA synthesis.

L'invention porte sur des protéines de fusion de transcriptase inverse stabilisée comprenant une transcriptase inverse thermostable reliée à une protéine stabilisatrice. La fixation de la protéine stabilisatrice à la transcriptase inverse thermostable stabilise la protéine de fusion et peut aider à sa purification, fournir une solubilité accrue, permettre un stockage plus long ou permettre à la protéine de fusion d'être utilisée dans des conditions plus rigoureuses telles qu'une température supérieure. La protéine de fusion de transcriptase inverse stabilisée peut également comprendre un liant entre la protéine stabilisatrice et la transcriptase inverse thermostable. Les protéines de fusion de transcriptase inverse stabilisée sont appropriées pour une utilisation dans des procédés d'amplification d'acide nucléique tels que la réaction en chaîne par polymérase de transcription inverse et d'autres applications mettant en jeu la synthèse d'ADNc.

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