Stabilized transient gene expression

Details Stabilized transient gene expression Stabilized transient gene expression

C - Chemistry, Metallurgy

12

N

C12N 15/87 (2006.01) A61K 31/131 (2006.01) A61K 31/136 (2006.01) A61K 31/137 (2006.01) A61K 31/192 (2006.01) A61K 31/198 (2006.01) A61K 31/205 (2006.01) A61K 31/4172 (2006.01) A61K 31/519 (2006.01) A61K 31/714 (2006.01) C12N 5/08 (2006.01) C12N 15/67 (2006.01) C12N 15/85 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2270137

This invention provides methods and agents for enhancing transient expression in eukaryotic cells. Also provided are a model system for achieving prolonged transient expression in solid tumors, a means for culturing hepatocytes without feeder cells or an extracellular matrix bonded to the substratum, and a mehtod for manipulating cellular metabolism to reduce the cells' consumption of glucose.

La présente invention concerne des procédés et agents permettant de renforcer l'expression transitoire dans des cellules eucaryotes. L'invention concerne également un système de modélisation servant à obtenir une expression transitoire prolongée dans les tumeurs solides, des moyens destinés à la culture d'hépatocytes sans cellules nourricières ou matrice extracellulaire liée au substrat, et un procédé de manipulation du métabolisme cellulaire visant à réduire la consommation de glucose par les cellules.

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