Stabilizing king crab amebocyte lysate

Details Stabilizing king crab amebocyte lysate Stabilizing king crab amebocyte lysate

C - Chemistry, Metallurgy

12

Q

150/16, 195/71

C12Q 1/44 (2006.01)

Patent

CA 1166985

ABSTRACT OF THE DISCLOSURE This invention relates to a method of stabilizing king crab amebocyte lysate against loss of potency. The method com- prises combining the lysate with an amount of heparin sufficient to stabilize the lysate against loss of potency but insufficient to inhibit the reaction between the lysate and endotoxins. The lysate is useful in an improved method for detecting endo- toxins in blood serum and/or plasma which utilizes the lysate in the presence of a substrate which has a selected colorimetric indicator bound to it. The improved stabilized lysate increases the accuracy of the tests for the detection of endotoxins which has not been possible in the past, due to the decrease in potency of the lysate of the prior art.

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