C - Chemistry – Metallurgy – 09 – K
Patent
C - Chemistry, Metallurgy
09
K
150/15, 150/3.2
C09K 3/00 (2006.01) C12Q 1/32 (2006.01) C12Q 1/50 (2006.01) C12Q 1/54 (2006.01) G01N 31/22 (2006.01)
Patent
CA 1043677
A B S T R A C T A stable, colored reference standard suitable for use in the deter- mination of enzymatic reactions capable of reducing the colorless 2-(p-iodo- phenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride to the red-colored 1- (p-iodophenyl)-5-(p-nitrophenyl)-3-phenylformazan is described. The refer- ence standard of this invention is an aqueous solution containing, in speci- fied amount,1-(p-iodophenyl)-5-(p-nitrophenyl)-3-phenylformazaan; serum albumin; an N,N'-dimethylformamide or a dimethylsulfoxide solvent; and iso- propyl alcohol. An inert bulking agent may be added to the aqueous colored standard and the solution obtained may then be lyophilized. The lyophilized form of the colored reference standard is stable for at least six months at 4°C. and 24°C. and can be easily reconstituted with water. The novel colored reference standard solution has an absorbence maximum at 500 nanometers. Enzymatic determinations in which the colored reference standard of this in- vention may be used include the determination of serum lactate dehydrogenase, creatine phosphokinase, glucose-6-phosphate dehydrogenase and the like.
236982
Adolf Paul K.
Carroll James J.
Na
Warner-Lambert Company
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