Stable composition comprising a nuclease and a phosphatase

Details Stable composition comprising a nuclease and a phosphatase Stable composition comprising a nuclease and a phosphatase

C - Chemistry, Metallurgy

12

N

C12N 9/22 (2006.01) C12N 9/16 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2403438

A composition containing a nuclease, preferably Exonuclease I, and a phosphatase, preferably Shrimp Alkaline Phosphatase, wherein the enzymes are combined in a single composition yet each enzyme retains significant functional activity over time. Combining Exonuclease I and Shrimp Alkaline Phosphatase into one composition allows simplified processing of amplified DNA to degrade residual primers and nucleotide triphosphates thereby facilitating subsequent DNA analysis.

L'invention concerne une composition contenant une nucléase, de préférence une exonucléase I, ainsi qu'une phosphatase, de préférence une phosphatase alcaline de crevette. Ces enzymes sont combinées dans une composition unique, chaque enzyme conservant toutefois une activité fonctionnelle importante au fil du temps. La combinaison d'exonucléase I et de phosphatase alcaline de crevette en une composition permet de réaliser un traitement simplifié d'ADN amplifié afin de dégrader les amorces résiduelles et les triphosphates nucléotidiques, ce qui facilite l'analyse subséquente d'ADN.

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