Stable hypoxia inducible factor-1 alpha and method of use

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) A61K 35/00 (2006.01) A61K 38/17 (2006.01) A61K 48/00 (2006.01) C07K 14/47 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2340328

Substantially purified stable human hypoxia-inducible factor-1.alpha. (sHIF- 1alpha) proteins and polynucleotides encoding stable human hypoxia-inducible factor-1.alpha. proteins are provided. A method is provided for treating a hypoxia-related tissue damage in a subject by administering to the subject a therapeutically effective amount of an sHIF- 1alpha protein or a nucleic acid encoding a stable HIF-1alpha protein. Formulations are provided for the administration of stable human hypoxia inducible factor-1.alpha. (HIF-1alpha) polypeptide or a polynucleotide encoding stable human hypoxia inducible factor-1alpha (HIF- 1alpha) to a patient having or at risk of having hypoxia- of ischemia-related tissue damage.

L'invention concerne des protéines, de facteur-1 alpha humain (sHIF-1alpha) stable, inductible en hypoxie et sensiblement purifié, et des polynucléotides codant pour ces protéines de facteur-1 alpha humain (sHIF-1alpha) stable, inductible en hypoxie. L'invention concerne également une méthode de traitement de lésions de tissus par hypoxie chez un sujet par l'administration à celui-ci d'une dose thérapeutiquement efficace d'une protéine sHIF-1alpha ou d'un acide nucléique codant pour une telle protéine. L'invention prévoit des formulations pour l'administration d'un polypeptide de facteur-1 alpha humain (HIF-1alpha) stable, inductible en hypoxie, ou d'un polynucléotide codant pour un facteur-1 alpha humain (HIF-1alpha) stable, inductible en hypoxie, à un patient présentant, ou sujet à, des lésions de tissus par hypoxie ou ischémie.

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