Stable integration of dna in bacterial genomes

C - Chemistry – Metallurgy – 12 – N

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C12N 15/74 (2006.01) C12N 1/21 (2006.01) C12N 15/55 (2006.01) C12N 15/63 (2006.01) C12N 15/75 (2006.01) C12N 15/76 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2070676

2070676 9109129 PCTABS00005 A bacterial cell which in its genone carries an integrated non-replicative DNA construct comprising (1) a DNA sequence of interest, (2) a DNA sequence which is homologous with a region of the genome of the cell, and (3) an origin of replication, said DNA construct lacking a functional gene coding for a factor required to initiate replication from said origin of replication.

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