Stable pura vectors and uses thereof

Details Stable pura vectors and uses thereof Stable pura vectors and uses thereof

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) A61K 39/02 (2006.01) A61K 39/112 (2006.01) A61K 39/12 (2006.01) C07K 14/245 (2006.01) C07K 14/445 (2006.01) C12N 1/04 (2006.01) C12N 1/21 (2006.01) C12N 9/00 (2006.01) C12N 15/68 (2006.01) C12N 15/70 (2006.01) C12N 15/74 (2006.01) C12N 15/90 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2067862

This invention pertains to a complementation system for the selection and maintenance of expressed genes in bacterial hosts. The invention provides stable vectors which can be selected and maintained by complementation of chromosomal deletion mutations of purA (adenylosuc- cinate synthetase), obviating the use of antibiotic resistance genes. This system is useful in production organisms during fermentation and in live vaccine bacteria, such as attenuated Salmonella typhi. This system allows for selection of chromosomal integrants and for selection and stable plasmid maintenance in the vaccinated host without application of external selection pressure.

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