Stable reagents and kits useful in loop-mediated isothermal...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2673772

Provided herein is a reagent preparation for loop-mediated isothermal amplification of nucleic acids comprising: at least one polymerase enzyme, a target- specific primer set, and dinucleotide triphosphates (dNTPs) in a single, dry format; wherein said reagent preparation is water soluble and stable above 4°C.

La présente invention concerne une préparation de réactifs pour amplification isotherme d'acides nucléiques induite par la boucle comprenant au moins une enzyme polymérase, un ensemble d'amorces spécifiques de la cible et des dinucléotides triphosphates (dNTP) sous une seule forme sèche, laquelle préparation de réactifs est hydrosoluble et stable à une température supérieure à 4 °C.

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