Stable transofrmation of maize cels by electroporation

C - Chemistry – Metallurgy – 12 – N

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C12N 15/90 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2097817

2097817 9212250 PCTABS00014 The present invention provides a method to increase the susceptibility of cultured Zea mays cells to stable transformation with recombinant DNA via electroporation, by pretreating the Zea mays) cells with certain pectin-degrading enzymes, so that the cells retain their ability to regenerate fertile, transgenic Zea mays) plants containing the DNA which is also heritable.

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