C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/0789 (2010.01) C12N 5/071 (2010.01) C12N 5/074 (2010.01) A61K 35/12 (2006.01) A61P 3/10 (2006.01) A61P 9/00 (2006.01) A61P 17/00 (2006.01) A61P 25/00 (2006.01) A61P 35/00 (2006.01)
Patent
CA 2544252
The present invention relates to methods and compositions concerning isolation of proliferating cells. In particular, the invention regards enrichment of stem cells in a mixture of stem cells and non-stem cells, wherein the non-stem cells may be differentiated cells. The invention exploits the non-adherent property of stem cells, as opposed to the adherent property of differentiating cells, by serially passaging the suspended cells in liquid media.
L'invention concerne des procédés et des compositions se rapportant à l'isolation de cellules à prolifération. L'invention concerne en particulier l'enrichissement de cellules souches dans un mélange de cellules souches et non-souches, les cellules non-souches pouvant être des cellules différenciées. L'invention exploite la propriété non-adhérente de cellules souches par opposition à la propriété adhérente de cellules différenciées en faisant passer séquentiellement les cellules suspendues en milieux liquides.
Bever Christopher T. Jr.
Goolsby James E.
Pessac Bernard M.
Trisler G. David
Borden Ladner Gervais Llp
U.s. Department Of Veterans Affairs
University Of Maryland Baltimore
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