Stem cell-derived retinal pigment epithelial cells

Details Stem cell-derived retinal pigment epithelial cells Stem cell-derived retinal pigment epithelial cells

C - Chemistry, Metallurgy

12

N

C12N 5/071 (2010.01) A61K 35/44 (2006.01) A61P 27/02 (2006.01)

Patent

CA 2684460

The present invention concerns RPE cells obtainable by directed differentiation from stem cell, particularly, human stem cells. It has been specifically found that culturing stem cells in the presence of one or more member of the TGF superfamily, such as Activin A) induced directed differentiation into mature and functional RPE cells. This was evidenced by the expression of markers specific to mature RPE cells, including MiTF-A, RPE65 or Bestrophin). In accordance with one particular embodiment, the cells are a priori cultured with nicotinamide (NA) which was found to augment the cells' response to the inductive effect of the one or more member of the TGF superfamily. The invention also provides methods of performing the directed differentiation, as well as methods for use of the resulting RPE cells.

Cette invention a trait à des cellules EPR qui peuvent être obtenues par différentiation orientée à partir de cellules souches, en particulier de cellules souches humaines. Des données ont montré spécifiquement que la mise en culture de cellules souches, en présence d'un ou de plusieurs membres de la superfamille des TGF, comme l'activine A) induit une différentiation orientée en cellules EPR matures et fonctionnelles. Ces données ont été mises en évidence par l'expression de marqueurs spécifiques des cellules EPR matures, y compris le MiTF-A, le RPE65 ou la bestrophine. Conformément à un mode de réalisation particulier, les cellules sont d'abord cultivées avec du nicotinamide (NA) qui s'avère augmenter la réponse cellulaire à l'effet inducteur d'un ou de plusieurs membres de la superfamille des TGF. L'invention concerne également des procédés de réalisation de la différentiation orientée, ainsi que des procédés utilisant les cellules EPR résultantes.

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