C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/85 (2006.01) C12N 5/10 (2006.01) C12N 5/16 (2006.01) C12N 15/11 (2006.01)
Patent
CA 2716609
A stem cell expression cassette, comprising a nucleic acid comprising a pluripotent stem cell specific promoter, and a tag sequence, wherein the pluripotent stem cell specific promoter and tag sequences are operatively linked, is provided. Also provided are methods of identifying and methods of selecting a pluripotent cell, using the stem cell expression cassette.
L'invention porte sur une cassette d'expression de cellules souches comprenant un acide nucléique comprenant un promoteur spécifique de cellules souches pluripotentes, et une séquence marqueur, le promoteur spécifique des cellules souches pluripotentes et les séquences marqueurs étant liés de façon fonctionnelle. L'invention porte également sur des procédés d'identification et des procédés de sélection d'une cellule pluripotente, à l'aide de la cassette d'expression des cellules souches.
Ellis James
Hotta Akitsu
Gowling Lafleur Henderson Llp
The Hospital For Sick Children
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