Stem cell gene targeting

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/11 (2006.01) A01K 67/027 (2006.01) C07H 21/00 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01) C12N 15/85 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2675952

The invention provides a method for generating a transgenic eukaryotic cell population having a modified human Rosa26 locus, which method includes introducing a functional DNA sequence into the human Rosa26 locus of starting eukaryotic cells. Also provided are targeting vectors useful in the method, as well as a cell population and a transgenic non-human animal comprising a modified human Rosa26 locus. Finally, the invention provides an isolated DNA sequence corresponding to the human Rosa26 locus.

L'invention propose un procédé pour générer une population cellulaire eucaryote transgénique ayant un locus Rosa26 humain modifié, lequel procédé comprend l'introduction d'une séquence d'ADN fonctionnel dans le locus Rosa26 humain de cellules eucaryotes de départ. Des vecteurs de ciblage utile pour le procédé sont également proposés, ainsi qu'une population cellulaire et un animal non humain transgénique comportant un locus Rosa26 humain modifié. Enfin, l'invention propose une séquence d'ADN isolé correspondant au locus Rosa26 humain.

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