Stem-like cells and method for reprogramming adult mammalian...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 5/071 (2010.01) C12N 5/079 (2010.01) A61K 31/401 (2006.01) A61K 38/05 (2006.01)

Patent

CA 2709566

A new use is provided for small molecule inhibitors of Oct4 and Sox 2 as a cellular reprogramming agent and a method of reprogramming adult mammalian somatic cells into stem-like cells is provided, using small molecule inhibitors of Oct4 and Sox 2 without the need of any material derived from embryos or fetuses, and without the need of potentially harmful transfecting vectors. Stem-like cells created by the present invention can be induced to differentiate into terminally differentiated adult somatic cells, such as, for example, neuronal cells.

La présente invention a trait à une nouvelle utilisation de petits inhibiteurs de molécule de Oct4 et de Sox 2 en tant qu'agent de reprogrammation cellulaire et à un procédé de reprogrammation de cellules somatiques de mammifères adultes en cellules souches utilisant de petits inhibiteurs de molécule de Oct4 et de Sox 2 sans qu'il soit nécessaire d'utiliser aucun matériau dérivé d'embryons ou de foeus et sans qu'il soit nécessaire d'utiliser de vecteurs de transfection potentiellement nocifs. Les cellules souches créées selon la présente invention peuvent être produites de manière à différencier des cellules somatiques d'adultes différenciées en fin de chaîne, telles que, par exemple, les cellules neuronales.

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