Sterilization indicator for liquid peracetic acid procedures

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/22 (2006.01) A61L 2/28 (2006.01)

Patent

CA 2362573

A sterilization indicator is useful for testing the effectiveness of sterilization procedures that disinfect objects by contacting them with a liquid sterilization procedure. The indicator includes an outer container having an open end and a cover material associated with the open end that is impermeable to liquids and bacteria. An enzyme-gel matrix is coated on a surface within the outer container that comprises a biologically inert polymeric gel and a source of an active enzyme dispersed within the gel. The enzyme has an activity that is correlated with the survival of at least one test microorganism that is commonly used to monitor the effectiveness of a sterilization procedure. A breakable ampoule within the outer container contains a substrate that is capable of reacting with any active enzyme remaining after the indicator has been subjected to a sterilization procedure to provide a detectable indication that the sterilization procedure was ineffective.

Un indicateur de stérilisation s'avère utile pour évaluer l'efficacité de procédures de stérilisation que l'on met en oeuvre pour désinfecter des objets par mise en contact desdits objets avec un produit stérilisant liquide. Ledit indicateur comporte un contenant externe ayant une extrémité ouverte et une matière de couverture associée à ladite extrémité ouverte, qui est imperméable aux liquides et aux bactéries. Une matrice enzyme-gel, qui recouvre une surface du contenant interne, contient un gel polymère biologiquement inerte et une source d'un enzyme actif dispersé au sein du gel. Ledit enzyme est doté d'une activité qui est corrélée à la survie d'au moins un micro-organisme d'essai qui est utilisé couramment pour contrôler l'efficacité d'un produit stérilisant. Une ampoule cassable, disposée à l'intérieur du contenant externe, contient un substrat qui est susceptible de réagir avec tout enzyme actif restant après mise en contact de l'indicateur avec un produit stérilisant dans le but de fournir une indication détectable relative à l'inefficacité du produit stérilisant.

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