Sterol glycosyl transferases

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/10 (2006.01) A01H 5/00 (2006.01) C07K 16/40 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01) C12P 33/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2268816

The invention relates to isolated DNA fragments or recombinant DNA constructs containing at least part of a sterol glycosyl transferase coding sequence. Substances used as sterols are either sterols in the strictest sense (cholesterol, ergosterol, beta-sistosterol, stigmasterol) or made from a skeletal structure of 5-alpha-cholesteran-3-beta-ol or 5-alpha-cholestan-3- alphol-ol (possibly modified by side-chains or double bonds in the ring systems). The invention also relates to DNA sequences, the use thereof to modify the content and/or structure of steryl glycosides and/or synthethic secondary products in transgenic organisms.

L'invention concerne des fragments d'ADN ou des produits de recombinaison d'ADN isolés comportant au moins une partie d'une séquence codant pour la stérol-glycosyl transférase. On utilise comme stérols des substances qui soit sont des stérols au sens strict (cholestérol, ergostérol, bêta-sitostérol, stigmastérol), soit sont constituées d'un squelette 5-alpha-cholestan-3-bêta-ol ou 5-alpha-cholestan-3-alpha-ol (éventuellement modifié par des chaînes latérales ou des liaisons doubles dans les systèmes cycliques). L'invention concerne d'autre part des séquences d'ADN, ainsi que leurs utilisations pour la modification de la teneur et/ou de la structure des stérylglycosides et/ou des produits secondaires synthétiques dans des organismes transgéniques.

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