Stevia rebaudiana with altered steviol glycoside composition

A - Human Necessities – 01 – H

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A01H 5/12 (2006.01)

Patent

CA 2325493

This invention relates to Stevia plants characterized by exhibiting a high level of total glycosides, and a high ratio between rebaudioside A and stevioside. Preferably the Stevia plants comprise a level of total steviol glycoside of at least 14 %, or of about 14.4 % to about 18.8 %, and the ratio of rebaudioside A:stevioside is of at least 9.1:1, or of about 9.3:1 to about 11.0:1. This invention also discloses a method for producing Stevia plants characterized in that the level of total steviol glycoside is at least of about 14 %, and the ratio of rebaudioside A:stevioside is of at least about 9.1:1, comprising selecting at least two intermating genotypes of Stevia each comprising a ratio of rebaudioside A:stevioside of at least about 9.1:1, and a total steviol glycoside level of at least about 14 %; allowing said at least two intermating genotypes to cross pollinate to produce a seed; collecting said seed; and growing said Stevia plant. Also disclosed are seeds and plants obtained from Stevia plants exhibiting high levels of total glycosides and high ratios between rebaudioside A to stevioside.

L'invention concerne de plantes Stevia caractérisées par un niveau élevé de glycosides totaux et un rapport élevé entre le rebaudioside A et le stévioside. Ces Stevia comprennent de préférence un niveau de glycoside de stéviol total d'au moins 14 %, ou compris entre 14,4 % et 18,8 % environ, et leur rapport rebaudioside A:stévioside est d'au moins 9,1:1, ou compris entre 9,3:1 et 11,0:1 environ. L'invention concerne également un procédé de production de Stevia caractérisées par un niveau des glycosides de stéviol totaux d'au moins 14 % environ, et un rapport rebaudioside A:stéviodside d'au moins 9,1:1. Ce procédé consiste à sélectionner au moins deux génotypes d'intercroisement de Stevia dont chacun présente un rapport rebaudioside A:stéviodside d'au moins 9,1:1 environ, et un niveau de glycosides de stéviol totaux d'au moins 14 % environ; à provoquer une pollinisation croisée de ces deux génotypes pour produire une semence; à récolter cette semence puis à cultiver les plantes de Stevia ainsi obtenue. L'invention concerne en outre les semences et les plantes obtenues à partir de Stevia présentant des niveaux élevés de glycosides totaux et des rapport importants entre le rebaudioside A et le stévioside.

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