Strain of rhodococcus rhodochrous ncimb 41164 and its use as...

Details Strain of rhodococcus rhodochrous ncimb 41164 and its use as... Strain of rhodococcus rhodochrous ncimb 41164 and its use as...

C - Chemistry, Metallurgy

12

N

C12N 9/88 (2006.01) C12N 1/20 (2006.01) C12P 13/02 (2006.01)

Patent

CA 2547393

A microorganism which is Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. A method of culturing the microorganism in a culture medium comprising urea or urea derivative is claimed. A nitrite hydratase obtainable from the microorganism is claimed. Also claimed is a process of preparing an amide from the corresponding nitrite wherein the nitrite is subjected to a hydration reaction in an aqueous medium in the presence of a biocatalyst selected from the group consisting of a microorganism which is a Rhodococcus rhodochrous strain NUMB 41164, a mutant thereof and a nitrite hydratase obtainable from Rhodococcus rhodochrous strain NCIMB 41164 or a mutant thereof. Also claimed is a method of storing the Rhodococcus rhodochrous NUMB 41164.

Cette invention concerne un micro-organisme qui est une souche de Rhodococcus rhodochrous NCIMB 41164 ou un mutant de celle-ci. Cette invention concerne un procédé de mise en culture de ce micro-organisme dans un milieu de culture comprenant de l'urée ou un dérivé de l'urée. Cette invention concerne également une nitrile hydratase pouvant être obtenue à partir de ce micro-organisme. Cette invention porte en outre sur un procédé de préparation d'un amide à partir du nitrile correspondant, le nitrile étant soumis à une réaction d'hydratation dans un milieu aqueux en présence d'un biocatalyseur sélectionné dans un groupe comprenant un micro-organisme qui est une souche de Rhodococcus rhodochrous NCIMB 41164, un mutant de celle-ci, et une nitrile hydratase pouvant être obtenue à partir de la souche de Rhodococcus rhodochrous NCIMB 41164 ou d'un mutant de celle-ci. Cette invention concerne enfin un procédé de conservation de Rhodococcus rhodochrous NCIMB 41164.

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