Strand displacement amplification using boronated nucleotides

Details Strand displacement amplification using boronated nucleotides Strand displacement amplification using boronated nucleotides

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2207065

.alpha.-Boronated deoxynucleoside triphosphates, when incorporated into a double-stranded restriction endonuclease recognition/cleavage site for a restriction endonuclease, induce nicking by the restriction endonuclease .alpha.-Boronated deoxynucleoside triphosphates (dNTP.alpha. BH3) are therefore useful as nucleotide analogs in SDA to produce the nickable hemimodified restriction endonuclease recognition/cleavage site required to sustain the amplification reaction.

Le désoxynucléoside triphosphate.alpha.boré incorporé dans un site de reconnaissance/coupure à double-brin par une endonucléase de restriction induit la coupure par cette dernière. Il (dNTP.alpha.BH3) est alors utile comme analogue de nucléotide dans l'amplification par déplacement de brin (SDA) pour produire le site de reconnaissance/coupure hémimodifié pouvant être coupé par une endonucléase de restriction, coupure nécessaire au maintien de la réaction d'amplification.

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