Streptomyces avermitilis gene directing the ratio of b2:b1...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/60 (2006.01) C12N 1/21 (2006.01) C12N 9/88 (2006.01) C12N 15/76 (2006.01) C12P 17/18 (2006.01) C12P 19/62 (2006.01)

Patent

CA 2381427

The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.

La présente invention concerne des molécules polynucléotidiques contenant des séquences nucléotidiques codant un produit génique aveC, lesquelles molécules polynucléotidiques peuvent être utilisées pour modifier le rapport ou la quantité d'avermectines de classe 2:1 produites dans des cultures de fermentation de S. avermitilis. La présente invention concerne également des vecteurs, des cellules hôtes et des souches mutantes de S. avermitilis dans lesquelles le gène aveC a été inactivé ou bien muté de manière à changer le rapport ou la quantité des avermectines de classe 2:1 produites.

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