Streptomyces microorganism useful for the preparation of...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/20 (2006.01) C12P 7/40 (2006.01) C12P 13/00 (2006.01) C12P 41/00 (2006.01)

Patent

CA 2227924

The invention disclosed relates to the biological production of substantially isomerically pure (R)-baclofen and structurally related compounds, from the racemic mixture of (R)- and (S)-isomers thereof, and to the isolation of a Streptomyces microorganism from Nature which is capable of preferentially metabolizing one of the isomers while showing minimal metabolic activity on the other isomer. A fermentation or bioconversion process using this microorganism or the cell-free enzymes derived therefrom for the biological resolution of a racemic mixture of (R)- and (S)-baclofen and structurally related compounds is also disclosed.

L'invention concerne la production biologique de baclofen-d essentiellement pur et de composés structurellement apparentés, à partir du mélange racémique des isomères d et l du baclofen, de même que l'isolement dans la nature d'un microorganisme du genre Streptomyces. Ce microorganisme est capable de métaboliser de préférence un des isomères tout en exerçant une activité métabolique minimale sur l'autre. Un processus de fermentation ou de bioconversion à l'aide de ce microorganisme ou des enzymes acellulaires qui en sont dérivés pour le dédoublement biologique d'un mélange racémique de baclofen-d et de baclofen-l et de composés structurellement apparentés est également dévoilé.

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