Streptomyces vectors for production of heterologous proteins

Details Streptomyces vectors for production of heterologous proteins Streptomyces vectors for production of heterologous proteins

C - Chemistry, Metallurgy

12

N

C12N 15/76 (2006.01) C07H 21/00 (2006.01) C07K 14/36 (2006.01) C07K 14/73 (2006.01) C07K 16/18 (2006.01) C12N 1/21 (2006.01) C12N 15/11 (2006.01) C12N 15/12 (2006.01) C12N 15/13 (2006.01) C12N 15/62 (2006.01) C12N 15/66 (2006.01)

Patent

CA 2087017

2087017 9200985 PCTABS00010 Nucleic acid sequences and DNA vectors useful for the production of CD4 chimeric proteins, as well as other heterologous proteins, in Streptomyces are disclosed. The nucleic acid sequences of the invention comprise the coding sequence for the signal peptide of the Streptomyces longisporus tyrosine inhibitor (LTI) gene operatively linked with a propeptide sequence consisting essentially of an amino acid sequence coding for from one to about 6 amino acids, the sequence of said amino acids selected to result in the formation in Streptomyces of a protein product having a homogeneous amino terminus after processing to remove said signal peptide formed on the protein product during synthesis of the protein product. In an alternative embodiment of the invention, the propeptide is omitted and the LTI signal peptide is operatively linked with a nucleic acid sequence coding for a heterologous protein which has been modified to code for the sequence lys-ala- at the 3' end. The invention also provides cells transformed with the nucleic acid sequences or vectors of the invention, and methods of using the nucleic acid sequences and vectors of the invention to produce heterologous proteins in Streptomyces.

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