Strong pcr primers and primer cocktails

Details Strong pcr primers and primer cocktails Strong pcr primers and primer cocktails

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/31 (2006.01)

Patent

CA 2550115

The invention disclosed relates to a PCR primer pair for amplication of chaperonin-60 (cpn60) targets having high G+C content and to a PCR primer "cocktail" to improve the representation of diverse sequences in chaperonin-60 based PCR product libraries derived from complex templates. In previous cpn60-based and 16S rDNA-based studies of mammalian intestinal microbiota, it has been observed that some classes of organisms such as the Actinobacteria, which are known through culture-based studies to be present in large numbers in these environments, are underrepresented or even absent from PCR product libraries. Using library sequence data and reference cpn60 sequence data from cpnDB, the chaperonin sequence database, we designed a pair of PCR primers which can be used alone for higher G+C content targets and, when used in combination with a previously developed degenerate, universal cpn60 primer pair, improve the representation of complex templates with high G+C content. We have validated these primers using a combination of traditional and quantitative real-time PCR on both artificially contracted complex templates and biological samples. The development and optimization of this primer cocktail represents a significant advance in our ability to generate cpn60 PCR product libraries which more closely represent the biodiversity in complex microbial communities.

Affiliated with

Also associated with

Rating

Strong pcr primers and primer cocktails does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Strong pcr primers and primer cocktails, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Strong pcr primers and primer cocktails will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1605948