G - Physics – 01 – N
Patent
G - Physics
01
N
167/44, 150/9, 1
G01N 33/53 (2006.01) C12Q 1/00 (2006.01) G01N 33/574 (2006.01)
Patent
CA 2002766
A b s t r a c t Altered saccharide structures on glycoproteins, glyco- lipids, poly- and oligosacchararides are detected and quantified in a body fluid by contacting the body fluid with a first ligand binding to the non-terminal constant portion of the saccharide structures and with a second ligand which either binds to a specifically altered ter- minal portion of the saccharide structures, but not to the normal terminal portion, or binds to the normal terminal portion of the saccharide structures, but not to the spe- cifically altered terminal portion, one of said ligands being immobilized on a solid phase, and the other being labelled, and thereafter detecting and, if desired, quantifying labelled immobilized glycocompounds. A glycosyltransferase enzyme producing such altered sac- charide structures is detected and quantifyed in a body fluid or a cell or tissue homogenate by contacting the body fluid or homogenate with an immobilized sugar chain which is an acceptor substrate for the enzyme and with a nucleotide-sugar which is a donor substrate for the en- zyme, adding a labelled ligand binding to the saccharide structure of the coupling product, and thereafter detect- ing and, if desired, quantifying labelled immobilized coupling product. A device for taking a sample of secretion via a body opening and for storing and processing the sample com- prises a container (14) and a rod (2) which is provided at one end portion thereof with an absorbing material (8) fixed to the rod (2) within the end portion in question, and which slidably extends through a cover means (12) at the other end portion, said cover means being adapted to be fixed in an opening of the container (14). Various kits for use in the above assays are also described.
Orntoft Torben F.
Swabey Ogilvy Renault
LandOfFree
Structural and enzymatic assays on glycocompounds, their use... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Structural and enzymatic assays on glycocompounds, their use..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Structural and enzymatic assays on glycocompounds, their use... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-2070137