Structural gene of pneumococcal protein

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/31 (2006.01) A61K 39/09 (2006.01) A61K 39/39 (2006.01) C07H 21/04 (2006.01) C07K 14/28 (2006.01) C07K 14/315 (2006.01) C07K 14/715 (2006.01) C12N 1/21 (2006.01) C12N 15/62 (2006.01) C12N 15/74 (2006.01) C12Q 1/68 (2006.01) G01N 33/569 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2104014

A purified pneumococcal surface protein A (PspA) comprises a truncated form of the PspA protein which is immunoprotective and contains the protective epitopes of PspA. The PspA protein is soluble in physiologic solution and tacks at least the cell membrane anchor region of the whole protein. The protein is formed by insertion-duplication of mutagenesis of S. pneumoniae with pspA gene and expression of the truncated protein into the growth medium.

Une protéine A purifiée de surface de pneumocoque (PspA) comprend une forme tronquée de la protéine PspA, laquelle est immunoprotectrice et contient les épitopes protecteurs de PspA. La protéine PspA est soluble dans une solution physiologique et est dépourvue d'au moins la région d'ancrage de membrane cellulaire de la protéine entière. La protéine est produite par insertion-duplication de mutagénèse de S. pneumoniae avec le gène pspA et par l'expression de la protéine tronquée dans le milieu de croissance.

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