Structure-based construction of human antibody library

C - Chemistry – Metallurgy – 40 – B

Patent

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C40B 50/02 (2006.01) C40B 30/02 (2006.01) C40B 30/04 (2006.01) G01N 31/00 (2006.01) G06F 17/50 (2006.01) G06F 19/00 (2011.01) C07K 16/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2443862

Methods and systems are provided for constructing recombinant antibody libraries based on three-dimensional structures of antibodies from various species including human. In one aspect, a library of antibodies with diverse sequences is efficiently constructed in silico to represent the structural repertoire of the verbrate antibodies. Such a functionally representative library provides a structurally diverse and yet functionally more relevant source of antibody candidates which can then be screened for high affinity binding to a wide variety of target molecules, including but not limited to biomacromolecules such as protein, peptide, and nucleic acids, and small molecules.

L'invention concerne des procédés et des systèmes permettant de construire des bibliothèques d'anticorps recombinants sur la base de structures tridimensionnelles d'anticorps de diverses espèces, y compris l'espèce humaine. Dans un aspect de cette invention, une bibliothèque d'anticorps à séquences diverses, construite in silico de façon efficace, représente la structure des anticorps de vertébrés. Cette bibliothèque fonctionnellement représentative constitue une source structurellement diverse et pourtant fonctionnement plus pertinente d'anticorps candidats. Ensuite, ces candidats peuvent être sélectionnés selon leur liaison de haute affinité à une grande variété de molécules cibles, comprenant notamment mais pas exclusivement des biomacromolécules telles que des protéines, des peptides et des acides nucléiques ainsi que des petites molécules.

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