Structure of g-protein (rgs4) and methods of identifying...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/47 (2006.01)

Patent

CA 2408930

The present invention provides a solution structure of free RGS4 determined using NMR techniques. The structure includes a G.alpha. binding site and allosteric bindingsite. The structural information provided can be employed to identify, select or design agonists and antagonists of RGS4 activity. The invention includes two dimensional and three dimensional models and representations of the structure of free RGS4 based upon structural coordinates that are provided that are useful in the methods described.

La présente invention concerne une structure de solution de RGS4 libre déterminée au moyen de techniques RMN. La structure comprend un site de liaison G.alpha. et un site de liaison allostérique. L'information structurelle fournie peut servir à identifier, sélectionner ou concevoir des agonistes et des antagonistes de l'activité RGS4. L'invention englobe des modèles et des représentations bidimensionnels et tridimensionnels de la structure de RGS4 libres basés sur des coordonnées structurelles fournies qui sont utiles dans le cadre des méthodes décrites.

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