Substantially pure enzyme-antibody conjugate preparations

Details Substantially pure enzyme-antibody conjugate preparations Substantially pure enzyme-antibody conjugate preparations

G - Physics

01

N

167/45

G01N 33/535 (2006.01) G01N 33/563 (2006.01)

Patent

CA 1302251

ABSTRACT OF THE DISCLOSURE A method for obtaining a substantially pure preparation of an enzyme-antibody conjugate having an enzyme component covalently coupled to a predetermined number of an antibody component where the molecular weight of the enzyme component is substantially greater than the molecular weight of the antibody component. The method involves the electrophoretic separation of the desired enzyme-antibody conjugate from an enzyme-antibody conjugate reaction mixture comprising, as separately migratable species, at least a free enzyme component and a population of enzyme-antibody conjugates comprising one or more of the enzyme component covalently coupled to one or more of the antibody component. The resulting conjugate preparations are substantially pure and are therefore particularly useful as labeled reagents in immunometric assays.

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