Substrate adhered polymyxin immunoassay device for...

G - Physics – 01 – N

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G01N 33/543 (2006.01) G01N 33/573 (2006.01) G01N 33/60 (2006.01) G01N 33/66 (2006.01)

Patent

CA 2017093

ABSTRACT OF THE DISCLOSURE An improved apparatus is disclosed for the detection of gram-negative bacterial lipopolysaccharide. The improved apparatus and process uses a minimum of expensive antibody. Polymyxin adheres to a substrate such as a plastic microtiter plate or cloth, either woven or non-woven. The polymyxin can then bind to lipopolysaccharide which may in turn be identified by standard enzyme, radio or fluorescent immunoassay. Non-woven polyester cloth is particularly suited for the purpose. The improved apparatus is particularly useful in the detection of bacterial pathogens in food.

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