Substrate and method for measuring the electrophysiological...

C - Chemistry – Metallurgy – 12 – M

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C12M 1/34 (2006.01) G01N 33/487 (2006.01)

Patent

CA 2480338

The present invention relates to a substantially planar substrate for use in patch clamp analysis of the electrophysiological properties of a cell membrane comprising a glycocalyx, wherein the substrate comprises an aperture having a rim, the rim being adapted to form a gigaseal upon contact with the cell membrane. The invention further provides a method of making such a substrate and method for analysing the electrophysiological properties of a cell membrane comprising a glycocalyx.

La présente invention concerne un substrat sensiblement plan prévu pour être utilisé dans l'analyse des propriétés électrophysiologiques d'une membrane cellulaire comprenant un glycocalyx, selon la technique de patch-clamp. Ce substrat comprend une ouverture dotée d'un rebord qui est adapté pour former un important scellement lors du contact avec la membrane cellulaire. L'invention traite également d'un procédé de fabrication d'un substrat et d'un procédé d'analyse des propriétés électrophysiologiques d'une membrane cellulaire comprenant un glycocalyx.

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