Substrate composition and method for solid phase urease...

Details Substrate composition and method for solid phase urease... Substrate composition and method for solid phase urease...

G - Physics

01

N

150/15, 167/44

G01N 33/543 (2006.01) C12Q 1/58 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2037521

P-1753 SUBSTRATE COMPOSITION AND METHOD FOR SOLID PHASE UREASE IMMUNOASSAY ABSTRACT OF THE DISCLOSURE A method for enzyme immunoassay of a ligand includes urease as a label. A bound fraction of ligand and antiligand conjugated to urease is formed on a solid support. The urease component of the bound fraction is contacted with a substrate composition for urease which includes a compound converted to ammonia by the urease, a tetrazolium salt and a pH dependent reducing agent which reduces the tetrazolium salt when the pH of the assay medium has been raised by the ammonia. The tetrazolium salt is reduced to a colored insoluble formazan which precipitates as a detectable spot on the support. The invention includes the substrate composition and a kit of materials for performing the assay.

Affiliated with

Also associated with

Rating

Substrate composition and method for solid phase urease... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Substrate composition and method for solid phase urease..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Substrate composition and method for solid phase urease... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1558501