Substrate for .alpha.-amylase and the preparation thereof

Details Substrate for .alpha.-amylase and the preparation thereof Substrate for .alpha.-amylase and the preparation thereof

C - Chemistry, Metallurgy

08

B

260/221, 260/220

C08B 31/00 (2006.01) C08B 33/00 (2006.01) C12Q 1/40 (2006.01)

Patent

CA 1110620

A B S T R A C T A substrate based upon particulate starch derivatised with deter- minable groups like reactive dyestuffs, for the determination of the .alpha.-amylase wherein the substrate consists of superficially cross-linked starch and/or amylase grains with a regulated, re- duced swellability, said grains having a diameter of from 0,01 to 0,20 mm. The preparation starts from non-swollen starch or amylase grains with a diameter of from 0,01 to 0,20 mm which are suspended in a solution with a content of difunctional cross- linking agent which is only sufficient for superficial cross- linking until the cross-linking agent is used up, whereafter the grains are derivatised with determinable groups.

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