Substrate for detection of mammalian 5-c-dna methyltransferase

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/48 (2006.01) C07H 21/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2180505

A substrate selective for detection of mammalian 5-C-DNA methyltransferase in the presence of bacterial 5-C-DNA methyltransferase, said substrate comprising oligomeric DNA which contains at least one 5-methylcytosine residue, and at least one cytosine or 5-fluorocytosine residue, each of which are followed in linkage to a guanine residue. The invention also includes a method for measuring the presence of mammalian 5-C-DNA methyltransferase which comprises contacting a sample containing 5-C-DNA methyl transferase with the substrate and also includes a method for inhibiting mammalian 5-C-DNA methyltransferase which comprises contacting a sample containing 5-C-DNA methyl transferase with the substrate.

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