Substrate for the quantitative determination of enzymes

C - Chemistry – Metallurgy – 12 – Q

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530/5.08, 150/3.

C12Q 1/56 (2006.01) C07K 5/083 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 1079167

A SUBSTRATE FOR THE QUANTITATIVE DETERMINATION OF ENZYMES ABSTRACT OF THE DISCLOSURE A substrate for the quantitative determination of enzymes in human and mammal body fluids as well as in animal cell extracts and glandular venoms of cold-blooded animals, which has the structure: R1 - Gly - Pro - X - NH - R2 wherein R1 represents hydrogen or a blocking acyl or sulfonyl group, R2 represents an aromatic hydrocarbon group which may carry substituents and X represents arginyl or lysyl, -NH-R2 being a chromogenic or fluorescent group capable of yield- ing a split product NH2-R2 the quantity of which can be measured by photometric, spectrophotometric or fluorescence- photometric methods.

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