Substrate formulation in 2-amino-2-methyl-1-propanol buffer...

C - Chemistry – Metallurgy – 12 – Q

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150/3.2

C12Q 1/42 (2006.01) G01N 33/535 (2006.01)

Patent

CA 1293677

Abstract A stable substrate formulation for an alkaline phosphatase assay includes 5-bromo-4-chloro-3-indolyl phosphate, nitro blue tetrazolium and a 2-amino-2- methyl-l-propanol buffer. Addition of MgC12 to this formulation promotes stability.

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