Substrates for hydrolases, a method for their preparation...

C - Chemistry – Metallurgy – 07 – H

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260/228, 530/5,

C07H 17/00 (2006.01) C07D 311/82 (2006.01) C07F 9/6533 (2006.01) C07F 9/655 (2006.01) C07H 17/02 (2006.01) C07H 17/04 (2006.01) C07H 17/075 (2006.01) C07H 21/00 (2006.01) C07K 5/00 (2006.01) C07K 5/083 (2006.01) C08B 30/18 (2006.01) C12Q 1/34 (2006.01)

Patent

CA 1283248

Abstract of the Disclosure: Chromogenic substrates of the formula A-O-B are described, in which A represents the radical in the ion A-O of a compound A-OH, whose absorbance is measured photometrically after hydrolysis of A-O-B, while 3 is the radical in a compound B-OH which makes the compound speci- fic for the reaction with a given enzyme, and where A-OH is a hydroxynitropyridine, a hydroxynitrotenzopyranone, a dibenzox(or thi)azine or a dibenzopyranone or a derivative thereof, and B is a sugar or sugar derivative, an amino acid or an oligopeptide or an inorganic acid. These compounds can be used for the detection and determination of hydrolases.

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