Substrates for immobilizing and amplifying dna,...

C - Chemistry – Metallurgy – 12 – M

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C12M 1/00 (2006.01) C12N 15/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2320462

Substrates for immobilizing DNA, etc. to give DNA libraries, etc.; and chips appropriately usable in amplifying DNA, etc. by PCR amplification. The PCR amplification is effected by using highly conductive solid substrates for immobilizing DNA. These substrates are surface-modified with chemicals having polar groups at the terminals. Use of the DNA-immobilized chips having DNA immobilized on these substrates makes it possible to amplify DNA within a short time in the PCR method.

La présente invention concerne des substrats permettant de bloquer l'ADN en vue de l'obtention d'échantillothèques d'ADN. L'invention concerne également des cristaux utilisables pour l'amplification d'ADN, notamment par amplification en chaîne par polymérase. Pour réaliser cette amplification en chaîne par polymérase, on utilise substrats solides à conductivité élevée permettant de bloquer l'ADN. Ces substrats ont été modifiés en surface par des agents chimiques portant des groupes polaires sur leurs terminaisons. L'utilisation de tels cristaux de blocage de l'ADN, où l'ADN reste bloqué sur les substrats, permet désormais de faire une amplification d'ADN en peu de temps selon la technique de l'amplification en chaîne par polymérase.

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