Sulfonamide resistance genes and their use

C - Chemistry – Metallurgy – 12 – N

Patent

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47/4, 167/5.1, 1

C12N 15/00 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) A01N 63/00 (2006.01) C12N 9/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2001935

Plant cells are transformed by: (i) transforming a plant cell whose growth is sensitive to inhibition by a sulfonamide or a salt thereof with a chimaeric gene comprising (a) a plant promoter, (b) a sulfonamide resistance gene having a sequence encoding a transit peptide fused to the 5'-end of the resistance gene and (c) a plant polyadenylation/terminator sequence; (ii) selecting a transformed plant cell whose growth is resistant to inhibition by a sulfonamide or salt thereof; (iii) optionally, regenerating from the transformed plant cell a genetically transformed plant which exhibit the said resistance; (iv) optionally, obtaining seed from the regenerated plant; and (v) optionally, propagating plants from the seed. The growth of weeds can be controlled at a locus where a transgenic plant obtained as above is being cultivated by applying to the locus an effective amount of a herbicide, such as asulam, which acts by inhibiting dihydropteroate synthase. The sulfonamide resistance gene can be also used as a selectable marker. The sequence of a sulfonamide resistance gene is presented.

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