C - Chemistry – Metallurgy – 09 – B
Patent
C - Chemistry, Metallurgy
09
B
C09B 19/00 (2006.01) C07H 21/00 (2006.01) G01N 33/533 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2407726
Fluorescent, sulfonated 3,7-diamino-[8,9]benzophenoxazine dyes are provided that are especially useful for labelling biopolymers and other substrates. The dye-labelled conjugates can be used in a variety of contexts, including cell surface assays employing intact, live cells and in nucleic acid detection methods. The new dyes are water soluble and can be conjugated to a variety of substrates, such as polynucleotides, nucleosides, nucleotides, peptides, proteins, antibodies, carbohydrates, ligands, particles and surfaces.
La présente invention concerne des colorants fluorescents sulfonés de 3,7-diamino-[8,9]benzophénoxazine particulièrement utilisés pour marquer des biopolymères et autres substrats. On peut utiliser les conjugués marqués par colorants dans divers domaines, notamment les dosages de surface cellulaire utilisant des cellules vivantes intactes, et dans des méthodes de détection d'acides nucléiques. Ces nouveaux colorants sont hydrosolubles et peuvent être associés à divers substrats, notamment les polynucléotides, les nucléosides, les nucléotides, les peptides, les protéines, les anticorps, les glucides, les ligands, les particules et les surfaces.
Yan Xiongwei
Yuan Pau Miau
Applera Corporation
Applied Biosystems Llc
Sim & Mcburney
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