Supplementation for embryo and/or cell manipulation

Details Supplementation for embryo and/or cell manipulation Supplementation for embryo and/or cell manipulation

C - Chemistry, Metallurgy

12

N

C12N 5/073 (2010.01) C12N 5/071 (2010.01) A01N 1/02 (2006.01) A61K 48/00 (2006.01) C12N 15/00 (2006.01) A61B 17/435 (2006.01) A61K 31/20 (2006.01) A61K 31/683 (2006.01) A61K 31/728 (2006.01)

Patent

CA 2603646

The invention relates to a system for increasing the quality and safety of embryo and cell manipulation media by supplementing said manipulation media with one or more of the following compounds: synthetic hyaluronan (sHA), phospholipids or unsaturated fatty acids that are obtained from soybeans (PLFA), thereby replacing other compounds which are normally added to embryo manipulation media and which can cause damage and/or contamination with viruses, prions, endotoxins, etc., which is important for the quality of the generated embryos or cells, both for the production of transgenic animals and animal production and for cell therapy or assisted reproduction. The inventive system reduces adhesiveness and increases viscosity, while retaining the fluidity of the medium, which is essential in micromanipulations such as ICSI, nuclear transfer, embryo biopsy, microinjection of cells into embryos during preimplantation stage or cell fusion.

L'inventions se rapporte à un système permettant d'améliorer la qualité et la sécurité des milieux utilisés dans les manipulations embryonnaires et cellulaires, par la supplémentation, dans le milieu de manipulation, d'au moins un des composés suivants : hyalurone synthétique (Has), phospholipides ou acides gras non saturés produits à partir des semences de soja (FAG), lesquels composés se substituent à d'autres composés qui sont normalement ajoutés aux milieux de manipulation embryonnaire et qui peuvent causer des dommages et/ou des contaminations avec un virus, des prions, des endotoxines, etc; ce qui est important pour la qualité des embryons ou des cellules générés, tant pour la production d'animaux transgéniques et pour la production animale que pour la thérapie cellulaire ou la reproduction assistée. Le système selon l'invention permet de diminuer l'adhésivité et d'augmenter la viscosité, tout en conservant la fluidité du milieu, laquelle fluidité est essentielle dans les micromanipulations comme l'ICSI, le transfert nucléaire, les biopsies embryonnaires, la microinjection de cellules dans des embryons à l'état préimplantatoire ou la fusion cellulaire.

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