C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
167/103, 195/33,
C12N 11/14 (2006.01) B01J 20/10 (2006.01) B01J 20/32 (2006.01) B01J 35/00 (2006.01)
Patent
CA 1130228
ABSTRACT An improved immobilized enzyme composite is prepared by subjecting a siliceous carrier to an initial treatment with alkali, then acid, then reacting the carrier with an organosilane. The organosilane may then be coupled through a covalent coupling agent with an enzyme. The alkali-acid treatment provide n exceptionally high number of hydroxyl groups per unit volume. so that the treated carrier is especially useful in the preparation of high performance immobilized enzyme compositions, particularly by multi-layering immobilization, providing a very high amount of enzyme activity per unit volume. Multi-layering is accomplished by successive steps of bonding a difunctional covalent coupling agent to a previously immobilized enzyme layer, then bonding an additional layer of enzyme to the coupling agent. This may be repeated to apply as many layers as desired.
352341
Ho Guan-Huei
Liao Chiang-Chang
Borden Foods Corporation
Macrae & Co.
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