Suppression of allergic reactions by transdermal...

A - Human Necessities – 61 – K

Patent

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A61K 39/39 (2006.01) A61K 9/70 (2006.01) A61K 38/16 (2006.01) A61K 39/106 (2006.01) A61K 39/108 (2006.01) A61K 39/35 (2006.01) A61K 39/36 (2006.01) A61K 45/06 (2006.01) A61K 47/48 (2006.01) A61M 37/00 (2006.01) A61P 37/08 (2006.01)

Patent

CA 2447795

The present invention discloses the use of the non-toxic cell-binding B subunit of CT (CTB), and holotoxin CT that is devoid of ADP-ribosylating activity, as adjuvants for enhancing transcutaneous immune response to a co- administered protein allergen. It was found that topical administration of CTB to mice induced serum antibody response against itself comparable to those evoked by CT, but was inefficient at promoting systemic antibody responses against an admixed prototype protein allergen. To the contrary co- administration of either CT or CTB with allergen led to vigorous antigen- specific T cell proliferative responses in lymph nodes draining the cutaneous site of administration and at distant systemic sites. Consistent with these observations, it was found that CTB selectively potentiated Th1-driven responses without affecting Th2-dependent responses. Cutaneously applied CT enhanced serum IgE responses to a co-administered allergen, while CTB partially suppressed epicutaneously induced IgE responses to the same allergen.

La présente invention concerne l'utilisation de sous unité de fixation de cellule bêta non toxiques de toxine de choléra (CT) (CTB), et de CT holotoxine intervenant dans l'activité ADP-rybosylante, comme adjuvants permettant de renforcer la réponse immune transcutanée à un allergène protéinique co-administré. On a découvert que l'administration topique de CTB à des souris induisait une réponse anticorps sérique dirigée contre elle-même comparable au potentiel évoqué par CT, mais que cette administration ne favorisait pas les réponses anticorps systémiques dirigées contre un allergène protéinique prototype mélangé. Au contraire, la co-administation de CT ou de CTB avec l'allergène conduit à une vigoureuse réponse proliférative de lymphocytes T spécifique de l'antigène dans les ganglions drainant le site cutané de l'administration et à des sites systémiques distants. En cohérence avec ces observations, on a découvert que la CTB potentialisait sélectivement les réponses induites par Th1 sans affecter les réponses dépendant de Th2. L'application cutanée de la CT renforce les réponses IgE sérique à un allergène co-administré, tandis que la CTB partiellement supprimée autour de la peau induit des réponses IgE à ce même allergène.

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