Suppression of non-specific nucleic acid amplification

Details Suppression of non-specific nucleic acid amplification Suppression of non-specific nucleic acid amplification

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2432016

The invention discloses methods of reducing background signal in nucleic acid amplification reactions by the use of primers in the case of isothermal amplification which include at least one modification selected from a nucleotide analogue, a hairpin loop at the 5'end of the primer, a ribonucleotide or a fluor or quencher. For more general nucleic acid amplification reactions the primer includes at least two of the modifications.

L'invention concerne des procédés permettant de réduire le signal de fond dans les réactions d'amplification d'acide nucléique par l'utilisation, dans le cas d'une amplification isothermique, d'amorces comprenant au moins une des modifications suivantes : un analogue nucléotidique, une boucle de type épingle à cheveu sur l'extrémité 5' de l'amorce, un ribonucléotide ou un fluor ou un extincteur (quencher). Pour les réactions d'amplification d'acide nucléique plus générales, l'amorce comprend au moins deux de ces modifications.

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