Suppressor trna transcription in vertebrate cells

Details Suppressor trna transcription in vertebrate cells Suppressor trna transcription in vertebrate cells

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12N 15/85 (2006.01) C12P 21/00 (2006.01) C07H 21/00 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2662755

This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in vertebrate cells. The components include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNA's/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in vertebrate cells are also provided.

Cette invention concerne des compositions et des méthodes permettant de produire des composants translationnels qui augmentent le nombre d'acides aminés codés génétiquement dans des cellules de vertébrés. Ces composants comprennent des ARNt orthogonaux, des aminoacyl- ARNt synthétases, des paires orthogonales d'ARNt/synthétases et des acides aminés artificiels. Sont également décrites des protéines et des méthodes de production de protéines avec des acides aminés artificiels dans ces cellules de vertébrés.

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