Surface display of recombinant proteins in lower eukaryotes

C - Chemistry – Metallurgy – 12 – N

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C12N 15/81 (2006.01) C07K 16/00 (2006.01) C07K 19/00 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01) C12N 15/80 (2006.01) C40B 40/02 (2006.01) C40B 50/06 (2006.01) G01N 33/567 (2006.01)

Patent

CA 2715212

Methods for display of recombinant proteins or protein libraries on the surface of lower eu-karyotes such as yeast and filamen-tous fungi are described. The meth-ods are useful for screening libraries of recombinant proteins in lower eu-karyotes to identify particular pro-teins with desired properties from the array of proteins in the libraries. The methods are particularly useful for constructing and screening antibody libraries in lower eukaryotes.

L'invention porte sur des procédés pour l'affichage de protéines recombinantes ou de bibliothèques de protéines sur la surface d'eucaryotes inférieurs tels qu'une levure ou des champignons filamenteux. Les procédés sont utiles pour cribler des bibliothèques de protéines recombinantes dans des eucaryotes inférieurs pour identifier des protéines particulières avec des propriétés désirées à partir du réseau de protéines dans les bibliothèques. Les procédés sont particulièrement utiles pour construire et cribler des bibliothèques d'anticorps chez les eucaryotes inférieurs.

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