Surface display of whole antibodies in eukaryotes

C - Chemistry – Metallurgy – 12 – N

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C12N 15/10 (2006.01) C07K 16/00 (2006.01) C12N 5/10 (2006.01) C40B 30/04 (2006.01)

Patent

CA 2730243

Methods for display of recombinant whole immunoglobulins or immunoglobulin libraries on the surface of eukaryote host cells, including yeast and filamentous fungi, are described. The methods are useful for screening libraries of recombinant immunoglobulins in eukaryote host cells to identify immunoglobulins that are specific for an antigen of interest.

La présente invention concerne des méthodes de présentation dimmunoglobulines entières ou de banques dimmunoglobulines recombinées à la surface de cellules hôtes eucaryotes, comprenant des levures et des champignons filamenteux. Les méthodes sont utiles pour le criblage de banques dimmunoglobulines recombinées dans des cellules hôtes pour identifier les immunoglobulines qui sons spécifiques dun antigène dintérêt.

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