C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/02 (2006.01) A61L 17/14 (2006.01) A61L 27/00 (2006.01) A61L 27/22 (2006.01) A61L 27/34 (2006.01) A61L 29/04 (2006.01) A61L 29/08 (2006.01) A61L 31/04 (2006.01) A61L 31/10 (2006.01) C07K 17/02 (2006.01) C07K 17/08 (2006.01) C07K 17/10 (2006.01) C12M 3/04 (2006.01) C12N 5/00 (2006.01)
Patent
CA 2066213
2066213 9105036 PCTABS00004 An absorbed protein-independent cell-adhesive surface is disclosed. The treated surface comprises a chemically derivatized material to which small peptides, having less than 12 amino acid residues and including YIGSR, RGD or REDV amino acid sequence are covalently linked to. The peptides of the present invention include a terminal glycine amino acid. Tresyl chloride activation of surface hydroxyl moieties provides the active surface sites by which a terminal glycine arm of a selected peptide attaches to form covalent bonds between the substrate and peptide. By way of example, surfaces which may be used in conjunction with the present invention include polymer, metal, and ceramic surfaces. The present methods also include a pretreatment method which provides hydroxyl moieties to surfaces devoid of readily available hydroxyl moieties. Methods of preparing the treated surfaces are also included in the present invention. Also included are surface-treated biomedical implant devices and cell culturing devices. The treated surface promotes an enhanced rate and an enhanced amount of cell adhesion to the surface, independent of media serum concentrations or other absorbed proteins. The invention further concerns polymeric substrates with a surface having physically interpenetrating water soluble polymer chains, and methods for production thereof.
Desai Neil P.
Hubbell Jeffrey A.
Massia Stephen P.
Blake Cassels & Graydon Llp
The Board Of Regents Of The University Of Texas System
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