Surfaces with reduced electroosmotic flow

G - Physics – 01 – N

Patent

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Details

G01N 27/447 (2006.01) B01D 57/02 (2006.01)

Patent

CA 2339235

An article of manufacture is provided that is useful in differentiating between solutes, such as during electrophoretic separations. An embodiment of the article is a capillary tube, that carries a polymer along the inner wall of the capillary tube. The polymer is effective to reduce undesired interactions and preferably includes a polylactam that is absorbed to the surface before the surface is exposed to the solutes. A preferred polylactam is poly(vinylpyrrolidone) with a molecular weight greater than about 1,000,000 daltons (weight-average).

L'invention concerne un article manufacturé utile dans la différenciation de solutés, par exemple lors de séparations électrophorétiques. Dans un mode de réalisation de l'invention, l'article est un tube capillaire le long de la paroi interne duquel un polymère est prévu. Le polymère est efficace pour réduire les interactions indésirables et comprend, de préférence, un polylactame qui est absorbé à la surface avant que cette dernière soit exposée aux solutés. Un polylactame préféré est le poly(vinylpyrrolidone) dont le poids moléculaire est supérieur à environ 1 000 000 daltons (indice de pondération).

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