Synthesis and use of organosilica particles

C - Chemistry – Metallurgy – 07 – F

Patent

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C07F 7/02 (2006.01) B01J 13/00 (2006.01) C07H 21/00 (2006.01) C08G 77/06 (2006.01) C08G 77/22 (2006.01) C08G 77/28 (2006.01) C08G 77/38 (2006.01) C08G 77/392 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2491110

Highly functionalized, porous organosilica particles and methods of their synthesis are described that employ high amounts of functional silane such as 3-mercaptopropyl trimethoxysilane. Silane particle diameters are controlled from less than 1 µm to over 100 µm. The particles have a high surface area due to their advantageous internal structures, which consist of large pores, typically up to 10 µm that are linked by small channels of typically about 20 nm diameter. Isothiocyanate modified fluorescent dyes can enter and react with thiol groups inside the pores. The invention also provides clear silica shells of controlled thicknesses to protect fluorescent signals and provide an independent parameter for distinguishing particle types based on light scattering off different sized particles. The particles are stable and useful for many purposes, particularly for optical bar coding in combinatorial synthesis of polymers such as nucleic acid, polypeptide, and other synthesized molecules.

L'invention porte sur des particules poreuses d'organosilice fortement fonctionnalisées et sur leur procédé de synthèse utilisant de grosses quantités de silane fonctionnel tel que le 3-mercaptopropyl triméthoxysilane. Le diamètre des particules de silane est maintenu entre moins de 1 µ et plus de 100µ. Les particules présentent une surface active élevée due à leur structure interne intéressante qui consiste en larges pores allant souvent jusqu'à 10µ, reliés par de petits canaux généralement d'environ 20 nm de diamètre. Les colorants fluorescents d'isothiocyanate peuvent pénétrer dans les pores et y réagir avec des groupes thiol. L'invention porte également sur des coquilles de silice claire d'épaisseur contrôlée destinées à protéger des signaux fluorescents et constituant un paramètre indépendant pour distinguer les types de particules sur la base de leur diffusion de lumière en fonction de leur taille. Lesdites particules sont stables et utilisables dans de nombreux domaines particulièrement pour les codes barres optiques dans la synthèse combinatoire de polymères tels que des acides nucléiques, des polypeptides, et autres molécules de synthèse.

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